Department of Prognosis of Conservative Treatment Effectiveness at the P.Hertsen Moscow Oncology Resaerch Institute

Отделение прогноза эффективности консервативного лечения МНИОИ имени П.А. Герцена

SERGEEVA NATALYA SERGEEVNA

POSITION: Head of the Department of Prognosis of Effectiveness of Conservative Treatment at the P. Hertsen Moscow Oncology Research Institute – branch of the Federal State Budgetary Institution “National Medical Research Radiological Centre” of the Ministry of Health of Russian Federation
ACADEMIC DEGREE: Doctor of Biological Sciences, Professor

Natalya Sergeevna Sergeeva – Head of Department
Irina Konstantinovna Sviridova – Senior Researcher
Tatyana Anatolyevna Karmakova – Senior Researcher
Nina Viktorovna Marshutina – Senior Researcher
Pavel Anatolyevich Karalkin – Senior Researcher
Valentina Aleksandrovna Kirsanova – Researcher
Suraya Abdulla Kyzy Akhmedova – Researcher
Yaroslav Dmitrievich Shansky – Researcher
Igor Igorevich Alentov – Researcher
Ekaterina Alekseevna Kuvshinova – Junior Researcher
Nina Tigranovna Makhmurova – Senior Laboratory Assistant
Ekaterina Gennadyevna Bashmurova – Laboratory Assistant
Irina Vasilievna Smirnova – Laboratory Assistant
Valentina Antonovna Bobrova – Laboratory Assistan
tElizaveta Konstantinovna Nezhurina – Junior Researcher
Olga Sergeevna Slukinova – Laboratory Assistant

The main directions of scientific research on the group of serological markers:
- development of clinical-diagnostic algorithms for the use of known and new tumor-associated serological, urological, and coprological markers in oncology for refined diagnosis, assessment of treatment effectiveness, disease prognosis, and preclinical detection of recurrences;
- conducting biomedical tests to evaluate the qualitative and functional characteristics of test systems designed for the determination of tumor-associated markers suitable for screening, refined diagnosis, disease prognosis, and monitoring of patients with malignant neoplasms;
- development of approaches to increase the sensitivity of laboratory diagnostics of solid tumors based on a combination of serological markers;
  determination of Epstein-Barr virus DNA levels in blood plasma for monitoring patients with nasopharyngeal cancer and EBV-associated gastric cancer;
- development of an algorithm for the use of the new urological marker uKIM-1 in patients with renal cell carcinoma (RCC) to improve the assessment of disease prognosis, monitoring treatment efficacy, and preclinical detection of RCC recurrence;
- creation of an algorithm for the use of uKIM-1 for predicting and early detection of nephrotoxicity from chemotherapy in cancer patients;
- investigation of the systemic inflammatory response to chemotherapy in cancer patients as a potential factor for predicting the effectiveness of specific treatments and their complications.
- Main areas of the research group’s work on the development and implementation of regenerative medicine methods in clinical practice
Development (in collaboration with scientific teams from several institutes of the Russian Academy of Sciences and Lomonosov Moscow State University) and preclinical testing of new domestic osteoplastic materials intended for reconstructive plastic surgery in oncology.
- Development of approaches for creating personalized constructs (using 3D printing methods) to replace bone defects in reconstructive plastic operations in oncology (in collaboration with the IEMET RAS and the Faculty of Materials Science of Lomonosov Moscow State University) and their preclinical testing.
- Development of a methodology for creating personalized functionalized 3D constructs with antitumor and antibacterial activity for the replacement of bone defects in reconstructive and plastic surgeries in oncology (in collaboration with FSBI IMET RAS and the Faculty of Materials Science of Lomonosov Moscow State University) and their preclinical testing.
- Development of a model of the bone metastatic niche for human breast cancer.
- Modeling angiogenesis in 3D in vitro cultures as a stage in the development of technology for producing prevascularized constructs for the replacement of organ and tissue defects.

“Serological tumor-associated markers in the diagnosis of malignant neoplasms and monitoring of cancer patients for the purpose of assessing treatment efficacy and preclinical detection of disease recurrence” within the framework of continuous medical and pharmaceutical education (CME)” / Interactive Educational Module (IEM) // Kaprin A.D., Sergeeva N.S., Marshutina N.V. https://fdpo.rsmu.ru/fdpo/
“Clinical significance of serological tumor-associated markers in solid tumors of main localizations”: educational module / Marshutina N.V., edited by Academician of the Russian Academy of Sciences A.D. Kaprin.Educational module “Modern concepts of serological tumor-associated markers and their role in oncology” / Marshutina N.V., edited by Academician of the Russian Academy of Sciences A.D. Kaprin. http://www.rosmedlib.ru/book/07-MOD-2023.htm
Serological Tumor-Associated Markers in Oncogynecology / Marshutina N.V., Alentov I.I. / In the book: Oncogynecology: National Guidelines. Edited by Kaprin A.D., Ashrafyan L.A., Stilidi I.S. Moscow – Geotar-Media Publishing Group LLC. – 2019.
Prognostic Significance of Serum Thymidine Kinase-1 Level in Evaluating the Effectiveness of Chemotherapy in Patients with Non-Hodgkin Lymphomas and Hodgkin Lymphoma (Medical Technology). Parilova N.K., Marshutina N.V., Tyurina N.G., Kaprin N.D., Parilov A.V. Moscow, 2016.
Diagnostic Efficiency of Enzyme Immunoassays Detecting Human Hemoglobin and Tumor Pyruvate Kinase in the Detection of Gastrointestinal Tumors (Medical Technology). N.V. Marshutina, E.V. Zenkina, N.K. Parilova, M.P. Solokhina

Biofabrication of a functional tubular construct from tissue spheroids using magnetoacoustic levitational directed assembly // Adv Healthc Mater. 2020. P. e2000721. doi: 10.1002/adhm.202000721
Magnetic levitational bioassembler for rapid formative biofabrication of 3D tissues and organs / Scaffold-free, Label-free, and Nozzle-free Magnetic Levitational Bioassembler for Rapid Formative Biofabrication of 3D Tissues and Organs // Int J Bioprint. 6(3). 2020. P.304. doi: 10.18063/ijb.v6i3.304.
Multiparametric analysis of tissue spheroids fabricated from different types of cells // Biotechnol J. 15(5). 2020. P.e1900217. doi: 10.1002/biot.201900217.
Formation of in situ magnesium-calcium-phosphate cements: from the synthesis of powder precursors in a single vessel to in vitro studies. In situ magnesium calcium phosphate cements formation: From one-pot powder precursors synthesis to in vitro investigations // J Bioactive Materials. 5(3). 2020. P.644-658.
Magnetic levitational bioassembly of a three-dimensional tissue construct in space. Magnetic levitational bioassembly of 3D tissue construct in space // Sci Adv. 6(29). 2020. P.eaba4174. doi: 10.1126/sciadv.aba4174.A breast cancer organoid model made it possible to identify potentially beneficial combinations of 3,3′-diindolylmethane and chemotherapeutic drugs.
Breast cancer organoid model allowed to reveal potentially beneficial combinations of 3,3′-diindolylmethane and chemotherapy drugs // Biochimie. 2020. S0300-9084(20)30265-0. doi: 10.1016/j.biochi.2020.10.007.
Biotechnology technology without the use of scaffolds using levitational assembly in a strong magnetic field. Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field. // Biofabrication. 12(4). 2020. P.045022. doi: 10.1088/1758-5090/ab7554.
Fabrication of three-dimensional calcium phosphate scaffolds for bone repair using magnetic levitational assembly. Fabrication of calcium phosphate 3D scaffolds for bone repair using magnetic levitational assembly // Sci Rep. 10(1). 2020. P.4013. doi: 10.1038/s41598-020-61066-3.
Physicochemical and biological characteristics of thin multilayer chitosan/κ-carrageenan films for surface modification of nitinol. Physicochemical and biological characteristics of chitosan/κ-carrageenan thin layer-by-layer films for surface modification of nitinol // Micron. 138. 2020. P.102922.
Hybrid tricalcium phosphate/hydrogel constructs functionalized with an antitumor drug for bone tissue regeneration. Hybrid Tricalcium Phosphate/Hydrogel Constructs Functionalized with an Antitumor Drug for Bone Tissue Regeneration // Inorganic Materials: Applied Research. 11(5). 2020. P. 1144-1152. DOI: 10.1134/S2075113320050160.
Genetically modified DR5-specific TRAIL variant DR5-B showed dual antitumor and protumoral effects in colon cancer xenografts and an improved pharmacokinetic profile. Genetically modified DR5-specific TRAIL variant DR5-B revealed dual antitumor and protumoral effect in colon cancer xenografts and an improved pharmacokinetic profile // Transl Oncol. 13(4). 2020. P.100762. doi: 10.1016/j.tranon.2020.100762.
3-D Cultivation of Human Thyroid Follicles in a Gel Based on Platelet Lysate // Cellular Technologies in Biology and Medicine. No. 2. 2020. P.104-111.
Validation of Threshold Decision Rules and Calculator for the VIZG Algorithm Designed to Clarify the Stage of Prostate Cancer Before Treatment // Oncourology. Vol. 16. 2020. P.33-46. DOI: 10.17650/1726-9776-2020-16-1-00-00.KIM-1 (Kidney Injury Molecule 1) in the Urine of Patients with Renal Cell Carcinoma // Oncourology. 16(3). 2020. P.21-28. https://doi.org/10.17650/1726-9776-2020-16-3-21-28.
The Influence of Co-Additive on the Sintering, Mechanical Properties, Cytocompatibility, and Digital Light Processing-Based Stereolithography of 3Y-TZP-5Al₂O₃ Ceramics // Materials (Basel). 13(12). 2020. P.2789. doi: 10.3390/ma13122789
Barrett’s Esophagus: Genetic Features of the Pathological Process // Experimental and Clinical Gastroenterology. 177(5). 2020. P.36–41. DOI: 10.31146/1682-8658-ecg-177-5-36-41.
Biological Markers of the Risk of Malignancy in Barrett’s Esophagus // Experimental and Clinical Gastroenterology. 177(5). 2020. P. 91–98. DOI: 10.31146/1682-8658-ecg-177-5-91-98.
Human platelet lysate preserves the osteogenic/adipogenic potential of mesenchymal stromal cells and maintains the integrity of their DNA in vitro. – Cells Tissues Organs. – 2019; 207:149–164. – DOI: 10.1159/000502813.
The effect of Al on the structure and in vitro behavior of hydroxyapatite nanopowders. – The Journal of Physical Chemistry B. 2019, 123, 43, 9143-9154. DOI 10.1021/acs.jpcb.9b08157.
Preparation and properties of copper-substituted hydroxyapatite powders and ceramics. – Inorganic Materials. Volume 55, Issue 10, 1 October 2019, Pages 1061-1067.
Results of the first stage of validation of the APhiGT algorithm for clarifying prostate cancer staging before the start of treatment. Oncourology. 2019; No. 2: pp. 72-82.

Method for determining the amount of doxorubicin released from functionalized calcium phosphate constructs / Shansky Y.D., Sergeeva N.S., Karalkin P.A., Poloznikov A.A., Kaprin A.D., Putlyaev V.I., Evdokimov P.V., Zuev D.M., Leontiev N.V. / Patent for invention No. 2722304, dated 28.05.2020.
Method for suppressing tumor growth with a genetically modified variant of the cytokine TRAIL / Gasparyan M.E., Yagolovich A.V., Dolgikh D.A., Kirpichnikov M.P., Artykov A.A., Karmakova T.A., Vorontsova M.S. / Patent for invention No. 2727059 C1, dated 17.07.2020.
Method for predicting the duration of the recurrence-free interval after completion of primary treatment in patients with ovarian cancer. / Alentov I.I., Sergeeva N.S., Marshutina N.V., Novikova E.G., Kaprin A.D. Registration number 2018111101. Patent for invention No. 2676702 dated January 10, 2019.
Method for predicting the clinical status of prostate cancer. Sergeeva N.S., Gitis V.G., Yurkov E.F., Pirogov S.A., Kaprin A.D., Alekseev B.Ya., Skachkova T.E., Marshutina N.V. Patent for invention No. 2664706 dated August 21, 2018.
Calculator for calculating multiparametric indicators for preoperative staging of prostate cancer. Skachkova T.E., Alekseev B.Ya., Kaprin A.D. Certificate of state registration of a software program. No. 2017616515. Date of state registration in the Register of Software Programs on June 8, 2017.
Method for predicting the extent of cytoreductive surgery in patients with advanced ovarian cancer after completion of the third course of neoadjuvant chemotherapy. Alentov I.I., Marshutina N.V., Novikova E.G., Kaprin A.D. Patent for invention No. 2671409 dated October 31, 2018.
Method for obtaining a culture growth supplement and a biocompatible hydrogel based on human platelet lysate. Shansky Ya.D., Sviridova I.K., Kirsanova V.A., Akhmedova S.A., Karalkin P.A., Kaprin A.D. Patent for invention No. 26657604 dated August 17, 2018.
Laboratory method for detecting common stages of lymphoproliferative diseases. Parilova N.K., Marshutina N.V., Tyurina N.G., Kaprin A.D. Patent for invention No. 2657604 dated 15.06.2018.
Method for obtaining a growth supplement based on platelet lysate from donor platelet mass for a medium to increase the cell mass of stem, progenitor, differentiated, and tumor cells. Shansky Y.D., Sviridova I.K., Kirsanova V.A., Akhmedova S.A., Kaprin A.D., Pirogov S.A. Patent for invention No. 2648162 dated 22.03.2018.
Hydrogel for obtaining composite materials with antibacterial activity for the replacement of bone-cartilage defects using 3D printing. Komlev V.S., Fedotov A.Yu., Teterina A.Yu., Barinov S.M., Sviridova I.K., Tyutkova Y.B., Karalkin P.A., Kirsanova V.A., Kuvshinova E.A., Kaprin A.D. Patent for invention No. 2632431 dated 04.10.2017.
Method for predicting the stage and aggressiveness of prostate cancer before surgery based on laboratory and clinical parameters. Gitis V.G., Yurkova E.F., Pirogov S.A., Kaprin A.D., Alekseev B.Ya., Skachkova T.E., Marshutina N.V., Mustafin A.G. Patent for invention No. 2614501 dated 28.03.2017.
Method for obtaining a composite three-dimensional scaffold for the replacement of bone-cartilage defects. Komlev V.S., Fedotov A.Yu., Teterina A.Yu., Zobkov Y.V., Tyutkova Y.B., Barinov S.M., Sergeeva N.S., Sviridova I.K., Kirsanova V.A. Patent for invention No. 2606041 dated 10.01.2017.
Method for predicting chemotherapy sensitivity in patients with lymphoproliferative disorders. Kaprin A.D., Parilova N.K., Tyurina N.G., Marshutina N.V. Patent No. 2593020. Bulletin No. 21, 27.07.2016.
Method for preoperative prediction of the stage and aggressiveness of prostate cancer. Gitis V.G., Yurkov E.F., Pirogov S.A., Kaprin A.D., Alekseev B.Ya., Skachkova T.E., Marshutina N.V., Mustafin A.G. Patent for invention No. 2611380 dated 21.02.2017.
Method for producing a growth supplement based on platelet lysate from donor platelet mass to a medium for cultivating the cellular mass of stem, progenitor, differentiated, and tumor cells. Sergeeva N.S., Shansky Ya.D., Sviridova I.K., Kirsanova V.A., Akhmedova S.A., Kaprin A.D., Pirogov S.A. / Patent for invention 2648162 dated 21.04.2016.