Radiation Biochemistry Department

Laboratory of Radiation Biochemistry

• Alexander Evgenievich Kabakov, Head of the Laboratory, Ph.D. in Biology
• Lyubov Vladimirovna Lyubina, Senior Researcher, Ph.D. in Medicine
• Olga Nikolaevna Matchuk, Researcher, Ph.D. in Biology
• Anna Olegovna Yakimova, Researcher, Ph.D. in Biology
• Galina Petrovna Bezyayeva, Researcher
• Vera Alekseevna Mosina, Researcher
• Larisa Viktorovna Panarina, Researcher
• Anna Vyacheslavovna Khokhlova, Researcher
• Anatoly Ivanovich Gunko, Lead Engineer

Post-Radiation Recovery Laboratory

• Zamulaeva Irina Alexandrovna, Head of Laboratory, Doctor of Biological Sciences, Professor
• Kiseleva Valentina Ivanovna, Senior Researcher, Candidate of Biological Sciences
• Selivanova Elena Ivanovna, Researcher, Candidate of Biological Sciences
• Abramova Maria Riksovna, Researcher
• Churyukina Kristina Alexandrovna, Junior Researcher
• Lipunov Nikita Mikhailovich, Junior Researcher
• Kiryushina Darya Yuryevna, Junior Researcher

Laboratory of Molecular and Cellular Radiobiology

• Tatyana Ilyinichna Ivanova, Head of the Laboratory, PhD in Biology
• Vera Alexeevna Khorokhorina, Senior Researcher, PhD in Biology
• Larisa Anatolyevna Dzikovskaya, Researcher, PhD in Biology
• Elena Sergeevna Degtyareva, Researcher

The Department of Radiation Biochemistry conducts fundamental and applied research in the field of experimental and clinical radiation biology and oncology at the molecular, subcellular, and cellular levels, including the following areas:

• Identification of patterns of radiation-induced damage in normal and tumor cells of humans and animals, and development of means and methods to modify radiosensitivity based on these findings;
• Investigation of the biochemical and molecular-biological mechanisms of cellular responses to damage in the short and long term after radiation exposure, including exposure to low doses;
• Development of new approaches and methods of personalized medicine necessary for assessing the effects of ionizing and non-ionizing radiation at the molecular and cellular levels;
• Development of biological (including molecular-genetic and virological) criteria for evaluating tumor processes to predict the effectiveness of radiation and combined treatment in cancer patients.

The scientific activity of the Laboratory of Radiation Biochemistry includes:

• Studying the molecular factors of cell resistance to radiation, chemical, and combined effects;
• Studying the biochemical mechanisms of normal and tumor cell death;
• Developing methods for radiosensitization of tumor cells.

The scientific activities of the post-radiation recovery laboratory include:

• Identifying the patterns and mechanisms of somatic mutagenesis (at the gene level) in cancer patients and individuals exposed to various types of radiation, as a basis for developing new methods in molecular epidemiology;
• Studying the radiobiological properties of tumor stem cells and searching for means of targeted impact on them;
• Investigating the mechanisms of photodynamic effects on tumor cells in experimental and clinical settings;
• Developing methods for assessing and predicting the individual response of tumor and normal cells to radiation, combined, and targeted treatments of malignant neoplasms.

The scientific activities of the Laboratory of Molecular and Cellular Radiobiology include:

• Experimental and clinical studies of the molecular and cellular mechanisms of oxidative stress and its role in the biological effects of ionizing and non-ionizing radiation;
• Investigation of DNA, chromatin, and biological membrane damage, and the development of new approaches to radiomodification;
• Analysis of gene polymorphisms as a factor of individual sensitivity to genotoxic effects, including anticancer therapy, and the development of new predictive biomarkers for radiation oncology and methods for assessing carcinogenic risk in molecular epidemiology.

1. Zamulaeva I.A., Selivanova E.I., Andreev V.G. Method for predicting radiosensitivity of malignant neoplasms of the upper respiratory tract. Patent for invention No. 273598 registered on 11.11.2020
2. Churyukina K.A., Zamulaeva I.A., Matchuk O.N., Zhuze A.L., Ivanov A.A. Method for reducing the number of breast stem cells. Patent for invention No. 2702910 registered on 14.10.2019
3. Zamulaeva I.A., Churyukina K.A., Zhuze A.L., Ivanov A.A. Method for reducing the clonogenic activity of breast cancer stem cells. Patent for invention No. 2700695 registered on 19.09.2019
4. Kiseleva V.I., Mkrtchan L.S., Lyubina L.V., Bezyayeva G.P., Panarina L.V., Lipunov N.M., Krikunova L.I., Zamulaeva I.A. Prevalence and genotype of high-risk human papillomavirus in patients of AF Tsyba MRNC with cervical intraepithelial neoplasia grade III (2010-2017). State registration certificate No. 2020620343 dated 25.02.2020
5. Zamulayeva I.A., Orlova N.V., Smirnova S.G., Abramova M.R., Andreev V.G. Database on the frequency of lymphocytes with mutations in T-cell receptor genes in the peripheral blood of patients with upper respiratory tract cancer. Certificate of state registration No. 2020620416 dated 05.03.2020
6. Zamulayeva I.A., Orlova N.V., Smirnova S.G., Selivanova E.I., Mkrtchan L.S., Krikunova L.I. Database on the frequency of lymphocytes with mutations in T-cell receptor genes in the peripheral blood of female residents of a region contaminated with radionuclides after the Chernobyl accident (Uzlovaya, Tula region). Certificate of state registration No. 2020620415 dated 05.03.2020
7. Zamulayeva I.A., Orlova N.V., Smirnova S.G., Mosina V.A., Maksyutov M.A. Database on the frequency of lymphocytes with mutations in T-cell receptor genes in the peripheral blood of nuclear industry workers (analysis in 2002-2004). Certificate of state registration No. 2020620431 dated 06.03.2020
8. Bogatyreva T.I., Afanasov A.O., Grivtsova L.Yu., Kuzmina E.G., Zamulayeva I.A., Mushkarina Falaleeva N.A., Terekhova A.Yu., Orlova N.V., Smirnova S.G. Content of CD4+ T-lymphocytes in the peripheral blood of newly diagnosed Hodgkin’s lymphoma patients before the start of treatment. Certificate of state registration No. 2020620346 dated 25.02.2020
9. Matchuk O.N., Kabakov A.E., Zamulaeva I.A. Content of 70 kDa heat shock protein in stem and non-stem breast cancer cells before and after γ-irradiation. Certificate of State Registration No. 2020620829 dated 22.05.202
10. Abramova M.R., Zamulaeva I.A. Viability of MCF-7 breast cancer cells after treatment with high molecular weight hyaluronic acid. Certificate of State Registration No. 2020621140 dated 06.07.2020
11. Zamulaeva I.A., Abramova M.R. Viability of HeLa cervical cancer cells after exposure to high molecular weight hyaluronic acid in vitro. Certificate of State Registration No. 2020621436 dated 14.08.2020
12. Selivanova E.I., Zamulaeva I.A., Andreev V.G. Relative content of tumor stem cells in biopsy material of upper respiratory tract cancer before treatment. State registration certificate No. 2020621266 dated 20.07.2020
13. Ivanova T.I., Dzikovskaya L.A., Khorokhorina V.A., Degtyaryova E.S., Ivanov S.A., Shegay P.V. Malondialdehyde (MDA) levels in blood plasma in carriers of ε2, ε3, ε4 haplotypes of the apolipoprotein E gene. State registration certificate No. 2020620613
14. Ivanova T.I., Khorokhorina V.A., Antoshchina M.M., Fesenko E.V., Krikunova L.I., Mkrtchyan L.S., Ivanov S.A., Shegay P.V. Database on the frequency of chromosomal rearrangements in blood lymphocytes of residents of radiation-contaminated areas in three regions of Central Russia 29-30 years after the Chernobyl accident. State registration certificate No. 2020620733
15. Ivanova T.I., Dzikovskaya L.A., Khorokhorina V.A., Krikunova L.I., Ivanov S.A., Shegai P.V. Malondialdehyde (MDA) Level in the Blood Plasma of Patients with Uterine Cancer Carrying APOE Gene Haplotypes ε2, ε3, ε4. State Registration Certificate No. 2020620709
16. Ivanova T.I., Dzikovskaya L.A., Khorokhorina V.A., Krikunova L.I., Ivanov S.A., Shegai P.V. Plasma Malondialdehyde (MDA) Level and Clinicomorphological Indicators in Patients Diagnosed with Uterine Cancer. State Registration Certificate No. 2020620867
17. Ivanova T. I., Sychenkova N. I., Khorokhorina V. A., Shinkarkina A. P., Konova M. V., Murzaeva A. V., Krikunova L. I., Shegay P. V., Ivanov S. A. APOE rs429358 polymorphism in patients diagnosed with ovarian cancer. State Registration Certificate No. 2020621162
18. Ivanova T. I., Potapov A. L., Ivanov S. A., Khorokhorina V. A., Zemskova D. V., Shegay P. V. rs4680 COMT polymorphism and clinical tumor parameters in colorectal cancer. State Registration Certificate No. 2020620960
19. Ivanova T.I., Sychenkova N.I., Khorokhorina V.A., Shinkarkina A.P., Murzaeva A.V., Krikunova L.I., Shegay P.V., Ivanov S.A. Polymorphism rs7412 of APOE in patients diagnosed with ovarian cancer. Certificate of state registration No. 2020621180
20. Mkrtchyan L.S., Zamulaeva I.A., Kiseleva V.I., Rubanovich A.V., Krikunova L.I. Prognosis of locally advanced cervical cancer (CxHPV-predictor). Certificate of state registration of a software program No. 2019617761 dated 20.06.2019

1. Kabakov A., Yakimova A., Matchuk O. Molecular Chaperones in Cancer Stem Cells: Determinants of Stemness and Potential Targets for Antitumor Therapy. Cells. 2020. V. 9. #4: 892.
2. Churyukina K.A., Zhuze A.L., Ivanov A.A., Zamulaeva I.A. Effects of dimeric bisbenzimidazoles and ionizing radiation on MCF-7 breast cancer stem cells. Biophysics. 2020.V. 65. № 1. P. 74-81.
3. I.A. Zamulaeva, E.I. Selivanova, V.I. Kiseleva, O.N. Matchuk, L.I. Krikunova, L.S. Mkrtchyan, A.D. Kaprin. Correlation of radiation response of cervical cancer stem cells with their initial number before treatment and molecular genetic features of papillomavirus infection. Bull Exp Biol Med. 2020. V. 170. №2. P. 241-245.
4. Zamulaeva I.A., Churyukina K.A., Matchuk O.N., Ivanov A.A., Saburov V.O., Zhuze A.L. Dimeric bisbenzimidazoles DB(n) in combination with ionizing radiation decrease number and clonogenic activity of MCF-7 breast cancer stem cells. AIMS Biophysics. 2020. V. 7. №4. P. 339-361.
5. O. N. Matchuk, I. A. Zamulaeva, E. I. Selivanova, L. S. Mkrtchyan, L. I. Krikunova, V. O. Saburov, A. A. Lychagin, G. Z. Kuliyeva, A. O. Yakimova, A. V. Khokhlova, S. A. Ivanov, A. D. Kaprin. Effect of Fractionated Low-LET Radiation Exposure on Cervical Cancer Stem Cells under Experimental and Clinical Conditions. Biology Bulletin. 2020. V. 47. №11. Р. 1471–1479.
6. T. I. Ivanova, A. L. Potapov, D. V. Zemskova, V. A. Khorokhorina, P. V. Shegai, S. A. Ivanov, and A. D. Kaprin. Evaluation of the association of rs4680 COMT polymorphism and clinical parameters of the tumor in colorectal cancer (a Pilot Study). Bull Exp Biol Med. 2020. V. 170. № 2. P. 254-257.
7. Izmest’eva O.S., Dzikovskaya L.A., Zhavoronkov L.P. Effect of rat exposure to moderate pressure oxygen atmosphere on resistance of erythrocytes against hemolysis. Bull Exp Biol Med. 2020. V. 169. № 2. P. 201-204.
8. Kiseleva V.I., Mkrtchyan L.S., Ivanov S.A., Lyubina L.V., Bezyaeva G.P., Panarina L.V., Krikunova L.I., Zamulaeva I.A. The presence of human papillomavirus DNA of integration is associated with poor clinical results in patients with third stage cervical cancer. Bull Exp Biol Med. 2019. T. 168, № 1. С. 87-91.
9. M. Mollaev, N. Gorokhovets, E. Nikolskaya, M. Faustova, A. Zabolotskya, O. Zhunina, M. Sokol, I. Zamulaeva, E. Severin, N. Yabbarov. Type of pH sensitive linker reveals different time-dependent intracellular localization, in vitro and in vivo efficiency in alpha-fetoprotein receptor targeted doxorubicin conjugate. International Journal of Pharmaceutics. 2019. Т. 559. С. 138-146.
10. I. A. Zamulaeva, E. I. Selivanova, O. N. Matchuk, L. I. Krikunova, L. S. Mkrtchyan, G. Z. Kulieva, A. D. Kaprin. Quantitative Changes in the Population of Cancer Stem Cells after Radiation Exposure in a Dose of 10 Gy as a Prognostic Marker of Immediate Results of the Treatment of Squamous Cell Cervical Cancer. Bull Exp Biol Med. 2019. V. 168. №1. P. 156-159.
11. Е. I. Sarapultseva, О. N. Matchuk, D. Uskalova, H. Markina, N. Savina, I. A. Zamulaeva И.А. The effects of electromagnetic field and γ-radiation on cell proliferation and regeneration in planarian Schmidtea mediterranea . IOP Conference Series: Materials Science and Engineering. 2019.V. 487. # 1. Article 012018. P. 1- 6.
12. E.I. Selivanova, I.A. Zamulaeva, L.S. Mkrtchyan, O. N. Matchuk, G.Z. Kulieva, L.I. Krikunova. Intratumoral subpopulation of cancer stem cells as predictive marker in cervical squamous cell carcinoma patients receiving chemoradiotherapy. International Journal of Molecular Medicine. 2019. V.44. Supplement 1. S55.
13. Matchuk O.N., Churyukina K.A., Zhuze A.L., Zamulaeva I.A. Radioresistance of breast cancer stem cells after single-dose and fractionated gamma-radiation exposure and radiosensitizing effect of dimeric bisbenzimidazoles in vitro. Journal of Medical Oncology and Therapeutics. 2019. V. 4: 38.
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