Radiation Biochemistry Laboratory

• Molecular diagnostics of infectious diseases (COVID-19, AIDS, hepatitis A, B, C, syphilis) using enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) – 3 people;
• Research on tumour stem cells (TSC) in different types of malignant neoplasms and development of methods for elimination and sensitisation of TSC – 2 people;
• Research on molecular mechanisms of adaptation and resistance of tumour cells to various types of anticancer therapy; development of methods for sensitising tumour cells to radiotherapy, chemotherapy and hyperthermia – 3 people;
• Participation in an international project in collaboration with colleagues from Serbia (Institute of Oncology and Radiology of Serbia, Belgrade, Serbia) on the study of anticancer properties of plant-derived polyphenol compounds.

“Method for reducing the number of mammary stem cells” Churyukina K.A., Zamulaeva I.A., Matchuk O.N., Juzhe A.L., Ivanov A.A.

Patent for invention No. 2702910 registered on 14.10.2019.

1. Radioresistance of breast cancer stem cells after single-dose and fractionated gamma-radiation exposure and radiosensitizing effect of dimeric bisbenzimidazoles in vitro. Matchuk O.N., Churyukina K.A., Zhuze A.L., Zamulaeva I.A. Journal of medical oncology and therapeutics. Proceedings of the International conference on “Oncology and cancer therapy”. March 18-19, 2019. London, Allied academies. 2019. V. 4, P. 38.
2. The effects of electromagnetic field and γ-radiation on cell proliferation and regeneration in planarian Schmidtea mediterranea. Е.I. Sarapultseva, О.N. Matchuk, D. Uskalova, H. Markina, N. Savina, I.A. Zamulaeva. Published under licence by IOP Publishing Ltd, IOP Conference Series: Materials Science and Engineering, 2019, V. 487, Number 1, 012018. 2019. V. 487, № 1. P. 012018.
3. Quantitative changes in the population of HeLa cervical cancer stem cells under the influence of fractionated gamma irradiation in vitro. Matchuk O.N., Zamulaeva I.A. Radiation and Risk. 2019. Vol.28, No. 2. Pp. 112-123.
4. Presence of integration of human papillomavirus type 16 DNA and the prognosis of an adverse outcome of stage III cervical cancer. V.I. Kiseleva, L.S. Mkrtchyan, S.A. Ivanov, L.V. Lyubina, G.P. Bezyayeva, L.V. Panarina, L.I. Krikunova, I.A. Zamulaeva. Bulletin of Experimental Biology and Medicine. 2019. Vol.168. No. 7. Pp. 100-105.
5. Fractionated exposure to low-ionising radiation on the population of cervical cancer stem cells under experimental and clinical conditions. Matchuk O.N., Zamulaeva I.A., Selivanova E.I., Mkrtchyan L.S., Krikunova L.I., Saburov V.O., Lychagin A.A., Kulieva G.Z., Yakimova A.O., Khokhlova A.V., Ivanov S.A., Kaprin A.D. Radiation Biology. Radioecology. 2019. Vol.59. No.5, Pp. 516-526.
6. Quantitative changes in the population of tumour stem cells after radiation exposure at a dose of 10 Gy as a prognostic marker of immediate outcomes. Zamulaeva I.A., Selivanova E.I., Matchuk O.N., Krikunova L.I., Mkrtchyan L.S., Kulieva G.Z., Kaprin A.D. Cellular Technologies in Biology and Medicine. 2019. No. 3. Pp. 191-194.
7. The presence of human papillomavirus DNA of integration is associated with poor clinical results in patients with third stage cervical cancer. Kiseleva V.I., Mkrtchyan L.S., Ivanov S.A., Lyubina L.V., Bezyaeva G.P., Panarina L.V., Krikunova L.I., Zamulaeva I.A. Bull Exp Biol Med. 2019. T. 168, № 1. С. 87-91.
8. Molecular Chaperones in Cancer Stem Cells: Determinants of Stemness and Potential Targets for Antitumor Therapy. Kabakov A., Yakimova A., Matchuk O. Cells. 2020 Apr 6;9(4):892. doi: 10.3390/cells9040892.PMID: 32268506.
9. Quantitative changes in the population of cancer stem cells after radiation exposure in a dose of 10 Gy as a prognostic marker of immediate results of the treatment of squamous cell cervical cancer. Zamulaeva I.A., Selivanova E.I., Matchuk O.N., Krikunova L.I., Mkrtchyan L.S., Kulieva G.Z., Kaprin A.D. Bull Exp Biol Med. 2019. V.168. №1. P. 156-159.
10. The relationship between the radiation response of cervical cancer stem cells and their initial quantity before treatment and the molecular-genetic characteristics of human papillomavirus infection. I.A. Zamulaeva, E.I. Selivanova, V.I. Kiseleva, O.N. Matchuk, L.I. Krikunova, L.S. Mkrtchan, A.D. Kaprin. Bulletin of Experimental Biology and Medicine. 2020. Vol. 170, No. 8. P. 215-220.
11. Hyperthermia as a method of radiosensitising tumour cells resistant to pharmacological radiosensitisers. A.V. Khokhlova, A.O. Yakimova, V.A. Mosina, E.I. Selivanova, A.E. Kabakov. Radiation Biology. Radioecology. 2020. Vol. 60, No. 5. P. 516-523.
12. Dimeric bisbenzimidazoles DB(n) in combination with ionising radiation decrease the number and clonogenic activity of MCF-7 breast cancer stem cells. I.A. Zamulaeva, K.A. Churyukina, O.N. Matchuk, A.A. Ivanov, V.O. Saburov, A.L. Zhuze. AIMS Biophysics. 2020. Vol. 7, No. 4. P. 323-345.
13. Enhanced radiosensitisation of tumour cells through combined hyperthermia and proteasome inhibitor treatment. Kabakov A.E., Mosina V.A., Khokhlova A.V. Radiation and Risk. 2020. Vol. 29, No. 4. Pp. 106-117.